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. 2017 Jan 23;12(1):e0170716. doi: 10.1371/journal.pone.0170716

Fig 5. Vinculin positive focal adhesion points disappear upon treatment with PSL1a.

Fig 5

(A) Confocal analysis of HeLa cells treated with 1 μg/ml PSL1a in serum-free medium for 30 min, fixed and stained for vinculin. PSL1a treatment leads to a strong reduction in the number and intensity of intracellular vinculin patches. Scale bar: 20μm. The quantification of vinculin intensity per cell upon treatment with PSL1a (1 μg/ml, 15–60 min) shows a significant reduction after 30 min. All data represent mean values +/- SEM from at least three independent experiments (90–120 cells per condition, per time point in each experiment, * P<0.05; *** P<0.001 compared to untreated control). (B) HeLa cells were transfected with RFP-vinculin and dynamic changes were analyzed by live cell imaging (see S1 File). PSL1a treatment leads to disappearance of vinculin patches (marked area) in a time-dependent manner. Scale bars: 10 μm.