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. 2017 Jan 23;12(1):e0170716. doi: 10.1371/journal.pone.0170716

Fig 6. PSL1a treatment inhibits protein and DNA synthesis in mammalian cells.

Fig 6

(A) HeLa, Hep-2, SKBR-3, PC3, and RPE cells were incubated with varying concentrations (5 ng/ml to 5 μg/ml) of PSL1a in serum-free medium for 4 h at 37°C. The level of protein synthesis was determined as described in the Experimental procedures. In contrast to all other tested cell lines, protein synthesis was not affected in non-cancer RPE cells. (B) HeLa cells were incubated with varying concentrations (5 ng/ml to 5 μg/ml) of PSL1a for 4 h at 37°C. The level of DNA synthesis, in parallel to the level of protein synthesis, was determined as described in Experimental procedures. All data represent mean values +/- SEM from at least three independent experiments (* P<0.05; ** P<0.01; *** P<0.001 compared to untreated control). (C) HeLa cells were treated with 5 μg/ml of PSL1a in serum-free medium at 37°C and the level of protein synthesis was measured at the indicated time-points. All data represent mean values +/- SEM from three independent experiments (* P<0.05; ** P<0.01 compared to untreated control).