Fig 4. Effect of MFE01 and T6SS mutants on MFP05 biofilm formation.

Biofilms were grown on a glass surface, for 48 h at 28°C, under a flow of LB medium. Biovolumes of fluorescent bacteria were determined by COMSTAT analysis after confocal laser scanning microscopy observation. P. fluorescens MFP05 bearing pSMC2.1 gfp, encoding green fluorescent protein, was co-cultured alone or with MFE01 or derivatives, in a 1:5 ratio. Each histogram represents the biovolume of fluorescent MFP05, relative to that of fluorescent MFP05 when MFP05-gfp is cultivated alone. Comparisons were made with the control MFP05-gfp; ** p-value < 0.01; * p-value < 0.05; ns = non-significant; n = 6 (the error bars represent the standard error of the mean).