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. 2017 Jan 23;12(1):e0170770. doi: 10.1371/journal.pone.0170770

Fig 5. Effects of hcp and tssC gene mutations on biofilm biovolume in P. fluorescens strain MFE01.

Fig 5

Biofilms were grown on a glass surface, for 48 h at 28°C, under a flow of LB medium. Bacteria were visualised with the Syto 9® green fluorescent nucleic acid stain. Biovolumes were determined by COMSTAT analysis, after confocal laser scanning microscopy observation. The values shown are biofilm biovolumes relative to that of wild-type MFE01. The data presented are the mean values for at least five independent experiments and the error bars represent the standard error of the mean. Statistical analyses were performed with non-parametric Mann-Whitney tests (two-tailed): ns indicates no significant difference in biovolume (p-value > 0.05) relative to the MFE01 biofilm, * and ** indicates significant difference in biovolume relative to the MFE01 biofilm: *p-value < 0.05, ** p-value < 0.01. ⧫ indicates a significant difference in biovolume (p-value < 0.05) relative to the MFE01ΔtssC biofilm. EV means empty pPSV35 (plasmid control).