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. 2017 Jan 23;12(1):e0170770. doi: 10.1371/journal.pone.0170770

Fig 7. Detection of AHLs with biosensor strains.

Fig 7

P.a indicates Pectobacterium atrosepticum 6276 strain, which produces C8- NAHLs, used as positive control. P.aΔexp1 means Pectobacterium atrosepticum 6276 mutant strain, which does not produce AHLs, used as negative control. A. Detection of short-chain AHLs with Chromobacterium violaceum CV026 (CV026). CV026 indicates C.violaceum CV026 strain, which produces vioalacein in contact with C4-C8 NAHLs, used as biosensor strain. Black arrow indicates violacein production by C.violaceum CV026 strain. LB plates were incubated for 72 h at 37°C (n = 3). B. Detection of long-chain AHLs with Agrobacterium tumefaciens NTI (ANTI1). ANT1 means Agrobacterium tumefaciens NT1 strain, which produces β-galactosidase in contact with C6-C12 NAHLs, used as biosensor strain. Red arrow indicates β-galactosidase production by Agrobacterium tumefaciens strain. LB plates containing X-Gal (40 μg/mL) were incubated for 72 h at 28°C (n = 3).