Table 2. Oligonucleotides used for this study.
Primer name | Primer sequence (54’-3’) |
---|---|
Muta1-hcp3-F | TCGACCACAACCATACAACA |
Muta2-hcp3-R | ATACTTCGTGGGTCCAGGTGATAAGTCCACTTCAGCTACCG |
Muta3-hcp3-F | ATCACCTGGACCCACGAAGTATCACCATGATCTTGTCTTCGC |
Muta4-hcp3-R | TGGATCAGCAAGTCCAGATC |
hcp3-EcoRI-F | TAATAAGAATTCACTTCATAACTAAGGAGCCA |
hcp3-XbaI-R | TAATAATCTAGAGTCGGTCATGCTCAGTTC |