Table 1. Nanoparticle tracking analysis of exosomes isolated from the commercial pooled, non-cancerous human serum using various techniques and serum volumes.
| Serum Volume | Isolation Technique | Mode/Diameter* (nm) (mean ± SEM) | Total Number of particles* (mean ± SEM) |
|---|---|---|---|
| 5 mL | miRCURY | 122 ± 10 | 7.3x1011 ± 3.0x1011 |
| ExoQuick | 111 ± 5 | 7.4x1011 ± 3.5x1011 | |
| TEIR | 105 ± 5 | 9.9x1011 ± 4.2x1011 | |
| UC | 134 ± 3 | 2.4x109 ± 1.1x108 | |
| 1 mL | miRCURY | 106 ± 3 | 1.5x1011 ± 2.6x1010 |
| ExoQuick | 109 ± 4 | 1.8x1011 ± 3.1x1010 | |
| TEIR | 112 ± 4 | 1.4x1011 ± 5.9x1010 | |
| UC | 133 ± 11 | 1.1x109 ± 2.0x108 | |
| 500 μL | miRCURY | 116 ± 5 | 4.5x1010 ± 8.9x109 |
| ExoQuick | 117 ± 8 | 6.6x1010 ± 1.2x1010 | |
| TEIR | 107 ± 3 | 1.0x1011 ± 2.6x1010 | |
| UC | 126 ± 2 | 4.6x108 ± 8.5x107 | |
| 250 μL | miRCURY | 118 ± 5 | 3.0x1010 ± 4.6x109 |
| ExoQuick | 119 ± 1 | 4.2x1010 ± 1.5x1010 | |
| TEIR | 122 ± 3 | 2.4x1010 ± 5.8x109 | |
| UC | 129 ± 7 | 2.9x108 ± 7.5x107 | |
| 100 μL | miRCURY | 119 ± 5 | 1.1x1010 ± 1.6x109 |
| ExoQuick | 126 ± 2 | 4.1x1010 ± 1.5x1010 | |
| TEIR | 125 ± 3 | 1.8x1010 ± 5.6x109 | |
| UC | 126 ± 4 | 1.2x108 ± 3.5x107 | |
| 50 μL | miRCURY | 117 ± 6 | 5.5x109 ± 8.5x108 |
| ExoQuick | 118 ± 2 | 6.5x109 ± 1.8x109 | |
| TEIR | 119 ± 4 | 6.6x109 ± 1.5x109 | |
| UC | 123 ± 4 | 7.0x108 ± 3.2x107 | |
| Average§ | miRCURY | 116 ± 3 | |
| ExoQuick | 117 ± 3 | ||
| TEIR | 115 ± 4 | ||
| UC | 129 ± 2 |
* Data included in this table represent the mean readings of three experimental replicates that were extracted, diluted, and measured separately. Data intervals represent the SEM.
§ Refers to the average diameter of the isolated particles by each technique from all starting volumes collectively. TEIR: Total Exosome Isolation Reagent from serum (Life Technologies, Carlsbad, CA); UC, ultracentrifugation; miRCURY: miRCURY Exosome Isolation Kit-Serum and Plasma (Exiqon, Woburn, MA), ExoQuick: Exoquick Serum Exosome Precipitation Solution (System Biosciences, Mountain view, CA).