Fig 3. 526A represses IAV-induced interferon stimulating genes (ISGs) and cytokine expression.

A549-PB1 cells were pre-treated with or without 75 μM 526A for four hours and subjected to PR8-PB1flank-eGFP IAV infection at an MOI of 1.0 for twelve hours. The expression of IFNβ, IFIT2, IP10 and IL6 was measured with RT-qPCR (Table 1). Error bars represent the variation range of duplicate experiments. Student’s t-test: *, p < 0.05; **, p < 0.01.