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Thermally destabilizing fragments decreased the dimerization affinity in CK2β P110D/V143D in homodissociation ITC experiments. (a) Homodissociation isotherm of CK2β P110D/V143D in 5% (v/v) DMSO. (b) Homodissociation isotherms of 4 and its analogues (4a and 4b). (c) Homodissociation isotherms of 16 and its analogues (16a–16d). The top and bottom panels of each ITC profile illustrate the raw calorimetric data and the integrated heats per injection, respectively.
