Table I.
Rates of oxygen consumption by mitochondria from ASE cells with different substrates*
| Substrate | Oxygen Uptake |
|---|---|
| (nmol oxygen/min mg protein) | |
| Pyruvate (5 mM) | 12 |
| Citrate (5 mM) | 22 |
| Pyruvate-Malate (5 mM / 2.5 mM) | 8 |
| Isocitrate (5 mM) | 16 |
| α-Ketoglutarate (10 mM) | 8 |
| Succinate (10 mM) | 6 |
| Fumarate (10 mM) | 7 |
| Glutamate (10 mM) | 10 |
| Glutamate-Malate (10 mM / 1 mM) | 10 |
| Malate (10 mM) | 9 |
| Oxaloacetate (5 mM) | ND |
| α-glycerophosphate (7.5 mM) | 14 |
| TMPD/Ascorbate (0.2 mM / 10 mM) | 33 |
| Acetoacetate (5 mM) | 11 |
| β-Hydroxybutyrate (5 mM) | ND |
| Octanoate (5 mM) | ND |
| Octanoyl-carnitine (20 μM) | 7 |
| Proline (10 mM) | 14 |
| Ornithine (10 mM) | ND |
| Glutamine (10 mM) | ND |
| Threonine (1 mM) | 5 |
| Methionine (1 mM) | ND |
| Lysine (1 mM) | ND |
| Glycine (1 mM) | ND |
| Leucine (1 mM) | ND |
| α-ketobutyrate (1 mM) | ND |
| α-ketoisovalerate (1 mM) | ND |
| α-keto-β-methylglutarate (1 mM) | ND |
| α-ketoisocaproate (1 mM) | ND |
*
Rates were measured during State 3 in the presence of 0.45 mM ADP. RCR were 4 or higher. Rates of State 4 were 2.3 ± 0.2 (mean ± SD). The numbers represent the mean with SD ≤ 10% mean. ND, not detectable.