Table IV.

Effect of inhibitors and uncouplers on oxidative phosphorylation in ASE cell mitochondria^*

Substrate and additions	Inhibition of State 3 rate (%)
Pyruvate-Malate (5 mM /2.5 mM)/5 μM rotenone	> 90
Pyruvate-Malate/ 10 mM malonate	50
Succinate (10 mM)/ malonate	50
α-glycerophosphate (7.5 mM)/ (1 μg/ml) Oligomycin	80
α-glycerophosphate/oligomycin/ 4 μM FCCP	~ 0
Glutamate/0.2 μM rotenone	92
α-glycerophosphate/ 5 μg/ml antimycin A	90
0.2 mM TMPD/10 mM ascorbate/ 1 mM NaN3	93
TMPD/ascorbate/ 1 mM KCN	100

^*All State 3 rates were evaluated with 0.45 mM ADP, with the exception of TMPD/ascorbate which was evaluated with 1 mM ADP.