A, B. NS-SV-AC or stably-transfected shRNA TLK1 cells were exposed to 8 Gy, and treated, or not, with GA for indicated times before they were analyzed by propidium iodide flow cytometry. Inset: Immunoblotting of cell lysates with antiserum to TLK1. C, D. Immunoblotting of irradiated NS-SV-AC cells with antibodies to phospho-histone H3 (Ser10), phospho-Chk1 (Ser317), and β-actin. UT: untreated; GA: gallic acid; IR: irradiation.