Figure 4.

Group-2 innate lymphoid cells (ILC2s) are required for the development of type 2 helper (T_H2) cells during primary infection with Heligmosomoides polygyrus. (a) Model of the experimental setup. Il7r^–/– mice were sublethally irradiated and reconstituted with Rorα^sg/sg bone marrow or 80% Rorα^sg/sg bone marrow with 20% wild-type (WT) or 20% Il4^–/– bone marrow. At 7 weeks postreconstitution, chimeric mice were infected with 200 H. polygyrus larvae and harvested at day 7 postinfection. (b) Representative fluorescence-activated cell sorting (FACS) plots of KLRG1⁺ cells within the Lin⁻Thy1.2⁺ gate and (c) frequency of Lin⁻Thy1.2⁺ ILCs and KLRG1⁺ ILC2s in the lamina propria (LP) of Rorα^sg/sg (–), Rorα^sg/sg:WT (WT), or Rorα^sg/sg:Il4^–/– (Il4^–/–) mice 7 days postinfection. (d) Total number of CD4⁺ TCRβ⁺ cells in the spleen of chimeric mice. (e) Frequency and (f) total number of cytokine-positive CD4⁺ CD44⁺ cells in the spleen of chimeric mice. Data are representative of two independent experiments with six to seven mice per group. *P≤0.05 using the Mann-Whitney test. FSC-H, forward side scatter of height; Lin, lineage; TCR, T-cell receptor.