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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: J Neurochem. 2017 Jan 12;140(3):421–434. doi: 10.1111/jnc.13913

Figure 4.

Figure 4

Overexpression of an autonomous Thr287 CaMKII autophosphorylation mutant does not alter EAAT1 and EAAT2 mediated [3H]-glutamate uptake in HEK293T cells. Shown are representative images of YFP Thr287Asp δCaMKII in A, mCherry EAAT1 transfected and B, mCherry EAAT2 transfected HEK293T cells. Images are viewed using the red channel (excit. 595 nm/emis. 620 nm) (left), green channel (excit. 490 nm/emis. 525 nm) (center) and merged (right). Average [3H]-glutamate uptake in C, EAAT1 (n=6, ± S.D.) and D, EAAT2 transfected cells (n=6, ± S.D.) normalized to transfected untreated HEK293T cells. UCPH-101 (10 μM) and TBOA (10 μM) were added to EAAT1 and EAAT2 transfected cells respectively as positive controls