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Competition ELISA against Dsg1 with Dsg4 or Dsg1 as a competitor. Three representative PF sera were incubated with serial dilution of concentrated culture supernatants containing hDsg4-His (open symbols) or hDsg1-His (closed symbols) and subjected to Dsg1 ELISA. The competition rate by Dsg4 increased in a dose-dependent fashion, although its plateau level was no more than 25%.
