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. 2004 Nov 15;114(10):1484–1492. doi: 10.1172/JCI20480

Figure 8.

Figure 8

Lack of in vitro digestion of Dsg4 by ETs. Human (A) and mouse (B) Dsg1(α)-His and Dsg4-His were incubated with ETA, ETB, ETD, or PBS alone (–) in vitro at 37–C for 1 hour. In both the human and mouse cases, Dsg1 (α) was cleaved by all of the ETs, while Dsg4-His was not digested by any of the ETs. The arrows and arrowhead indicate intact recombinant Dsg proteins and the cleaved protein, respectively. The bars indicate the molecular weight standards (from top to bottom: 100, 75, 50, 37, and 25 kDa). (C) Amino acid sequence alignment of human Dsg1, mouse Dsg1α, human Dsg4, mouse Dsg4, and human Dsg3 around the cleavage site of ETs (arrowhead). Amino acid residues that are conserved in human or mouse Dsg4 are shaded.