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. 2004 Nov 15;114(10):1433–1443. doi: 10.1172/JCI21753

Figure 5.

Figure 5

Influence of polycystin-2 expression. (A) When coexpressed in Cos-7 cells with full-length polycystin-2 (red), p-200 (green) is excluded from the nucleus and localizes instead to intracellular membranes whose distribution resembles that of the ER. (B) Coexpression of polycystin-2 with p200 decreases the AP-1 activation induced by p200 by a factor of about 2.5 (top). The level of expression of the p200 and pc-2 constructs was determined by Western blotting (bottom). Polycystin-2Ø (pc-2Ø) refers to a construct that lacks the domain of polycystin-2 that interacts with polycystin-1. (C–H) The CTT of polycystin-1 (stained with Mex-46; C and F) is detected in the nuclei of renal tubular epithelial cells from mice expressing a reduced level of polycystin-2 (C–E, Pkd2+/–; F–H, Pkd2WS25/–); nuclei were detected with Hoechst 33342 (D and G). (E and H) Superposition of Hoechst (blue) and Mex-46 (red) staining. Original magnifications, ×100 (A) and ×750 (C–H).