Skip to main content
. Author manuscript; available in PMC: 2017 Jan 23.
Published in final edited form as: Hum Gene Ther. 2008 Mar;19(3):279–286. doi: 10.1089/hum.2007.0136

FIG. 1.

FIG. 1

Immunoreactive hEpo and hGH detected in media and lysates of transfected HEK 293 cells. Forty-eight hours after transfection either with pAC-CMV-hGH, pAC-CMV-hEpo, or pAC-CMV-hEpo-hGH, media were collected and subjected to (A) hEpo ELISA or (B) hGH ELISA. Media from hEpo- and hGH-transfected cells were immunoreactive only in their respective ELISA. Media from hEpo–hGH-transfected cells reacted positively with both hEpo and hGH ELISAs. Data are presented as means ± SEM (n = 3). Western blots (C) from lysates of cells transfected with plasmids encoding hEpo–hGH reacted with both anti-hEpo (left) and anti-hGH (right) antibodies. hGH and hEpo have apparent molecular masses of ~22 and ~34 kDa, respectively. The predicted size of the chimeric hEpo–hGH fusion protein is ~56 kDa. The arrows to the right indicate the molecular masses of simultaneously electrophoresed protein standards. See Materials and Methods for additional details.