Fig. 1. CXCL9 and CXCL10 are expressed in the skin during vitiligo and form gradients within the tissue.
a. REX3 mice were used as hosts in the vitiligo model as shown. b. Representative image depicting a REX3 mouse with vitiligo on the tail. c. Sample images from week 7 mice depicting chemokine clouds (10x z stack). d. Quantification of reporter expression versus depth in skin. CXCL10 is expressed more superficially than CXCL9 (3 representative mice with similar tail scores at week 7, mean ± SEM; dashed line indicates dermal-epidermal junction; 2-way ANOVA significant for depth only). e. Example of punctate CXCL10 reporter expression in a vitiligo mouse. f. Representative z stack from a naïve mouse shows very little reporter expression (10x, auto-fluorescent hairs visible; each tick on axes = 0.1mm).