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Expression of CsWRI1s and WRI1 downstream targets in developing Arabidopsis seeds. Total RNA was isolated from developing seeds 6–8 days after flowering of wri1-3 mutant and transgenic plants (T2). The isolated RNAs were subjected to RT-PCR analysis. The EIF4A1 gene was used to determine RNA quality and quantity. PI-PKβ1, At5g52920. BCCP2, At5g15530.
