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. 2017 Jan 24;8:34. doi: 10.3389/fpls.2017.00034

FIGURE 9.

FIGURE 9

Fatty acid content present in triacylglycerols (A) and total lipids (B) of N. benthamiana leaves transiently overexpressing three CsWRI1 isoforms. Agrobacterium harboring empty vector (EV), CsWRI1A. CsWRI1B, or CsWRI1C was infiltrated to 5-week-old N. benthamiana leaves and the N. benthamiana plants were further incubated for 5 days. Total lipids were extracted from N. benthamiana leaf disks including the injection region and analyzed by gas chromatography or fractionated on thin layer chromatography. The eluted TAG fractions were transmethylated and the fatty acid methyl esters were analyzed by gas chromatography. Each value is the mean ± SE of three independent measurements. Data were statistically analyzed using Student’s t-test (P < 0.01).