FIGURE 2.

Time- and dose-dependent extracellular alkalinization of potato suspension cells in the presence of elicitors. (A) Effect of PAMP and DAMP elicitors on the extracellular pH changes. ^∗OGA concentrations were 1 μg/mL (black) and 10 μg/mL (gray pattern). (B) Effect of 2 μM of potato systemin and 4 μg/mL of chitin oligomers mixture on extracellular pH over 20 min. (C,D) Alkalinization effect of potato systemin and chitin mixture, respectively, at various concentrations. The pH was recorded after 15 and 5 min of suspension cells incubation with systemin and chitin, respectively. Histograms show mean ± SE of three replicates in three independent experiments.