Figure 2.

Representative growth curve for all bioreactor cultivations of Y. lipolytica. Strain DSM 3286 cultivated in a bioreactor containing 500 mL of nitrogen-limited media at pH 4.5 with 100 g^*L⁻¹ glycerol as carbon source and 3.1 g^*L⁻¹ of (NH₄)₂SO₄ as sole nitrogen source. Nitrogen-limitation triggers the formation of citric acid and polyols after 12 h, when the entire ammonium from the media is consumed.