Table 3.
Comparison of Y. lipolytica strains screened under different pH conditions in bioreactor batch cultivations for the production of polyols and citric acid on pure glycerol as sole carbon source.
| Strain name | pH | CDW (g/L) | Mannitol (g/L) | Yield Mannitol (g/g) | Erythritol (g/L) | Yield Erythritol (g/g) | Citric Aicd (g/L) | Yield Citric Acid (g/g) | Isocitric Acid (%) | References | Nitrogen Source |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Y. lipolytica A UV'1 | 3.00 | – | 27.6 | 0.16 | 59.3 | 0.35 | – | – | – | Tomaszewska et al., 2012 | 2 g*L−1 NH4Cl + 1 g*L−1 yeast extract |
| Y. lipolytica A-15 | 3.00 | – | 23.0 | 0.16 | 28.0 | 0.19 | – | – | – | Tomaszewska et al., 2012 | 2 g*L−1 NH4Cl + 1 g*L−1 yeast extract |
| Y. lipolytica Wratislavia K1 | 3.00 | – | 12.8 | 0.12 | 42.0 | 0.22 | – | – | – | Tomaszewska et al., 2012 | 2 g*L−1 NH4Cl + 1 g*L−1 yeast extract |
| 3.00 | 19.3 | 15.1 | – | 40.7 | 0.28 | 2.6 | – | – | Tomaszewska et al., 2014a | 3 g*L−1 (NH4)2SO4 + 1 g*L−1 yeast extract | |
| 4.50 | 23.7 | 6.1 | – | 32.2 | 0.22 | 45.9 | 0.31 | – | Tomaszewska et al., 2014a | ||
| 5.50 | 17.3 | 6.3 | – | 26.5 | 0.18 | 65.0 | 0.43 | 5.8 | Tomaszewska et al., 2014a | ||
| 5.50 | 15.7 | 8.3 | – | 30.2 | – | 53.3 | 0.34 | 4.8 | Rywińska et al., 2010 | 3 g*L−1 NH4Cl + 1 g*L−1 yeast extract | |
| Y. lipolytica A-101 | 5.50 | 17.1 | 4.9 | – | n.d.# | – | 66.5 | 0.44 | 21.1 | Rywińska et al., 2010 | 3 g*L−1 NH4Cl + 1 g*L−1 yeast extract |
| Y. lipolytica DSM 3286 | 3.00 | 14.1 | 23.5 | 0.23 | 15.1 | 0.15 | 2.2 | 0.02 | 13.3 | This study** | 3.1 g*L−1 (NH4)2SO4 |
| 4.50 | 11.2 | 12.3 | 0.12 | 5.9 | 0.05 | 34.1 | 0.34 | 8.2 | |||
| 5.50 | 11.7 | 6.3 | 0.06 | 3.9 | 0.04 | 44.0 | 0.44 | 8.6 | |||
| Y. lipolytica DSM 21175 | 3.00 | 20.9 | 25.4 | 0.27 | 1.0 | 0.01 | 0.6 | 0.01 | n.d. | This study** | 3.1 g*L−1 (NH4)2SO4 |
| 4.50 | 21.7 | 25.1 | 0.26 | 0.6 | 0.01 | 4.2 | 0.04 | 13.1 | |||
| 5.50 | 22.4 | 24.7 | 0.25 | n.d. | – | 5.1 | 0.05 | 14.9 |
n.d.—not detected.
Erythritol has been reconsumed after glycerol depletion.
Maximum values produced after 48 and 72 h for polyols and citric acid, respectively.