Figure 5.

IAN effects on the wild type as well as on NIT1ox, nit1-3, and NIT2RNAi plants. (A) Seven day-old seedlings grown under sterile conditions with the indicated amounts of IAN in the media given in μM. Scale bar = 2 mm. (B) Assessment of the differential effect of IAN on root elongation in the studied genotypes. In order to examine the sensitivity toward IAN in the media without the bias of initially shorter primary roots, the influence of IAN is expressed in relative terms. To do so, the length of the longest primary root of each genotype grown under control conditions was set to a value of 1 and all other roots of the corresponding genotype were expressed relative to this value. The concentration of the IAN content in the plates is indicated. For each condition and genotype at least 15 seedlings were measured. The data represent means ± SE. Asterisks indicate significant differences between the corresponding WT control and the different tested genotypes under the given condition (^*p < 0.05, ^**p < 0.01; Student's t-test).