Figure 2.

CBP_NZ7 inhibits LPS-induced recruitment of inflammatory monocytes and dendritic cells (DC) into skin. Bone marrow cells from eGFP donor mice were transfused intravenously into recipient mice 24 h before intradermal injection of LPS (1 μg per site) with and without CBP_NZ7. In addition each mouse received injections of CBP only and PBS for a total of four injections per mouse (each mouse within the group received LPS only, LPS + CBP, CBP only, PBS only and n = 3 animals per group for one experiment). Twenty-four hour later, the recruitment of eGFP/Gr1⁺/CD11b⁺ cells and eGFP/CD11c⁺/MHCII⁺ cells into the skin were analyzed. The bar represents the mean ± SD of 3 combined experiments (biological replicates) where n = 9 mice. Asterisks indicate results that are significantly different (p < 0.01; paired Students t-test).