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. 2017 Jan 24;8:18. doi: 10.3389/fphys.2017.00018

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Copyright © 2017 Svendsen, Isidor, Praetorius and Leipziger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Basolateral ATP (100 μM) stimulates very similar increases of [Ca²⁺]_i in the absence and presence of the P2X receptor blocker oATP. (A) Original trace of normalized fluo-4 fluorescence (F/F₀) without oATP (50 μM) pre-incubation. (B) Original trace of normalized fluo-4 fluorescence (F/F₀) with oATP (50 μM) pre-incubation. (C) Summary of all results. The [Ca²⁺]_i plateau was measured after 2 min of ATP application.