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. 2017 Jan 24;8:18. doi: 10.3389/fphys.2017.00018

Figure 5.

Figure 5

No effect of L-NAME (100 μM) on ATP-induced inhibition of NaCl absorption in isolated perfused mouse mTAL. (A) Representative original trace of the basolateral ATP effect in mouse mTAL during the application of basolateral L-NAME (100 μM). Recording of the electrical parameters Vte (transepithelial voltage) and ΔV0 (voltage deflection) in freshly dissected and perfused mouse mTAL used to quantify transport changes. (B) Summary: calculated equivalent short-circuit current (I'sc) of n = 6 experiments before, during (after 1 min) and after ATP wash-out (2 min after) in the presence of L-NAME. (n = 6, *p < 0.05 by student's T-test). (C) Summary time curves of I'sc values with basolateral ATP (100 μM) given at time point 5 min for 2 min in the absence and presence of L-NAME (n = 6). (D) Summary of ATP-inhibited I'sc with and without L-NAME pre-incubation (n = 6) and with L-arginine/L-NAME pre-incubation (n = 6, *p < 0.05 by student's T-test).