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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: J Invest Dermatol. 2016 Oct 7;137(2):475–483. doi: 10.1016/j.jid.2016.09.023

Figure 3. KIND1-loss sensitizes keratinocytes to UVB-induced DNA-damage.

Figure 3

(a) Immunofluorescent staining of γH2AX. Human keratinocytes transfected with siCon or siKIND1 were treated with UVB for 5 seconds (6.8mJ/cm2), and then immunostained for γH2AX followed by detection with an Alexa-555-conjugated secondary antibody. γH2AX [orange], nuclei [blue, Hoechst]. (b) Quantification of γH2AX-positive cells. Graph represents average percentages of γH2AX-positive cells + SD. (c) Immunofluorescent staining with a FITC-conjugated antibody against CPD [green]. Bright field images were shown below each corresponding CPD image. (d) Quantification of CPD-positive cells. Graph represents average percentages of CPD-positive cells + SD. P-values of <0.05 were obtained with student T-test. Scale bars: 50 μm.