. Author manuscript; available in PMC: 2017 Jan 24.

Published in final edited form as: Biochemistry. 2016 Oct 5;55(41):5781–5789. doi: 10.1021/acs.biochem.6b00801

Table 3.

The melting temperature (T_m) and melting enthalpy (ΔH_m) measured by DSC of GCC, G^5mCC, and G^5hmCC core DNA constructs in solution. The entropy of melting (ΔS_m) for each construct is calculated from the T_m and ΔH_m by ΔS_m = ΔH_m/T_m, with the assumption that at the melting temperature, the concentrations of folded and denatured DNA are equal and, thus, ΔG_m = 0⁵¹.

	Duplex			Junction			J–D

DNA Core:	T_m (°C)	ΔH_m (kcal/mol)	ΔS_m (cal/mol K)	T_m (°C)	ΔH_m (kcal/mol)	ΔS_m (cal/mol K)	ΔT_m (°C)
GCC	68.01 ± 0.11	81.7 ± 0.7	239 ± 2	73.50 ± 0.08	109.5 ± 1.4	316 ± 4	5.49 ± 0.14
G^5mCC	70.06 ± 0.10	68.1 ± 1.0	198 ± 2	75.20 ± 0.06	98.7 ± 1.0	284 ± 3	5.14 ± 0.12
G^5hmCC	65.03 ± 0.09	66.7 ± 0.5	196.9 ± 1.6	70.60 ± 0.05	97.3 ± 0.7	283 ± 2	5.57 ± 0.10