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. Author manuscript; available in PMC: 2017 Jan 24.
Published in final edited form as: Biochemistry. 2016 Oct 5;55(41):5781–5789. doi: 10.1021/acs.biochem.6b00801

Table 4.

Thermodynamic stabilization of G5mCC and G5hmCC junction cores relative to the GCC junction core. The enthalpic, entropic, and overall free energies (at 25°C) for each modified construct are listed with the values from the parent construct subtracted (ΔΔH25°C, ΔΔS25°C, and ΔΔG25°C, respectively). Values reflect stabilization per interaction at each crossover strand; therefore, each complete junction structure is stabilized by twice the tabulated energies.

DNA Core ΔΔH25°C (kcal/mol) ΔΔS25°C(cal/mol·K) ΔΔG25°C (kcal/mol)
G5mCC - GCC −2.1 ± 1.0 −6 ± 3 −0.22 ± 0.13
G5hmCC - GCC −1.5 ± 0.9 −5 ± 3 −0.01 ± 0.12