Skip to main content
. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Hepatology. 2016 Dec 19;65(2):661–677. doi: 10.1002/hep.28894

FIGURE 3. Fibrotic liver CD4+Foxp3+ T cells suppress CD8+ T cell response.

FIGURE 3

(A) CFSE labeled OT-I CD8+ T cells were cultured in the presence of irradiated CD8-depleted splenocytes pulsed with 3ug/mL ovalbumin peptide (SIINFEKL) 257–264 or unloaded (5:1 ratio, CD8: stimulators). CD4+CD25+ T cells (>80% Foxp3+) were added from indicated sources in a 1:1 ratio with CD8+ T cells. After 5 days of culture, proliferation was assed by CFSE dilution on live, non-auto-fluorescent CD8+ T cells, (left). Activation was assessed by CD25 and CD44 expression (middle and right). (B) ELISA was performed on supernatants to quantify IFNγ production from indicated wells (# denotes IFNγ concentration below 15pg/mL in supernatant). Data are representative of 3 independent experiments with pooled CD4+CD25+ T cells from indicated organs (n=3–5 animals per group). Statistics were calculated using one-way ANOVA of duplicate wells from at least 3 independent experiments (*p<0.05); error bars reflect SEM.