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. Author manuscript; available in PMC: 2017 Oct 18.
Published in final edited form as: Biochemistry. 2016 Oct 7;55(41):5818–5831. doi: 10.1021/acs.biochem.6b00834

Figure 7.

Figure 7

Stopped-flow transient kinetic time courses of the CmlA:CmlP reaction monitored at 340 nm. (A) Reaction of WTR with CmlPAT and O2. With O2 (black), with CmlPAT and O2 (red), and with CmlPAT~L-PAPA and O2 (blue). (B) Reaction of E377DR CmlA with CmlPAT and O2. With O2 (black), with CmlPAT and O2 (red), and with CmlPAT~L-PAPA and O2 (blue). Reactions were performed in 50 mM HEPES pH 7.5 at 4.5 °C and contained 75 μM CmlA ± 100 μM CmlPAT and 0.95 mM O2.