. Author manuscript; available in PMC: 2017 Oct 18.

Published in final edited form as: Biochemistry. 2016 Oct 7;55(41):5818–5831. doi: 10.1021/acs.biochem.6b00834

Table 4.

Transient kinetic reaction parameters^a

	τ₁⁻¹ (s⁻¹)	τ₂⁻¹ (s⁻¹)
WT^R + O₂	0.016 ± 0.001
WT^R + CmlP_AT + O₂	0.034 ± 0.01	0.010 ± 0.001
WT^R + CmlP_AT~L-PAPA + O₂	14 ± 2	2.0 ± 0.3
E377D^R + O₂	0.89 ± 0.03	0.12 ± 0.07
E377D^R + CmlP_AT + O₂	1.0 ± 0.1	0.21 ± 0.07
E377D^R + CmlP_AT~L-PAPA + O₂	1.3 ± 0.1	0.27 ± 0.1
WT^R + CmlP_AT~L-Tyr + O₂	12 ± 1	2.1 ± 0.1
WT^R + CmlP_AT~D-Tyr + O₂	0.14 ± 0.01	0.036 ± 0.001

All values were determined at 4.5 °C. Errors represent one standard deviation. Reactions contained 75 μM chemically reduced CmlA ± 100 μM CmlP_AT and 0.95 mM O₂.