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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Osteoarthritis Cartilage. 2016 Oct 13;25(2):332–340. doi: 10.1016/j.joca.2016.10.007

Figure 5. TGF-β1 stabilizes SOX9 protein in bovine chondrocytes.

Figure 5

In A, mRNA was collected from bovine chondrocytes treated with vehicle or TGF-β1 for the indicated amounts of time. SOX9 mRNA levels were determined by qPCR (REST, no statistically significant differences, n = 5). In B, protein was collected from bovine chondrocytes that were treated with either vehicle or TGF-β1 for 4 or 6 hours. SOX9, pSMAD3, and SMAD2/3 protein levels were determined by Western blot (n = 3). α-Tubulin was used as a loading control. In C, new protein synthesis was inhibited with cycloheximide. Cells were subsequently treated with either vehicle or TGF-β1 for up to 8 hours. Protein was isolated at specified time points, and the levels of SOX9 protein were determined by Western blot (n = 3). pSMAD3 = phosphorylated SMAD3, CHX = cycloheximide.