Table 1.
Primer sequences for qPCR
| Gene | Efficiency % | Forward primer | Reverse primer |
|---|---|---|---|
| bGAPDH | 110 | GGG TCA TCA TCT CTG CAC CT | GGT CAT AAG TCC CTC CAC GA |
| bPAPSS2 | 114 | TGC CAT CTT CCC ATC TCC CAT GTT | ACA GGT CTC TCT TGG TCT CAG GAT |
| bPRG4 | 101 | TGC CCT GAC TTC AAG AAG GAA TGC | CCA TAA TCG GAA CAG CAC TTG CCA |
| bPTHrP | 134 | CGG TTA TTA TTT CGG AGG AGG C | CCT CTC GCT CTG GGG ACT TAT |
| bSOX9 | 117 | CCG GCT CCG ACA CCG AGA ACA | CCA GCG TCC AGT CGT AGC CCT |
| mPpia | 99 | CGC GTC TCC TTC GAG CTG TTT G | TGT AAA GTC ACC CTG GCA CAT |
| mPapss2 | 98 | CCC GTG ATG GAG TCA ACA TGA G | GTG CTT TGC AGT GGG TGT TCC |
| mAcan | 96 | AGG TTG CTA TGG TGA CAA GG | TGG AAG GTG AAT TTC TCT GGG |
| mSox9 | 102 | AGG AAG TCG GTG AAG AAC GG | TGG AAG GTG AAT TTC TCT GGG |