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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Hepatology. 2016 Dec 24;65(2):616–630. doi: 10.1002/hep.28912

Figure 3. Adult onset Nampt over-expression is sufficient for regenerative phenotypes.

Figure 3

Mice carrying a Cre-inducible allele of Nampt infected with AAV-Cre (denoted as NAAVcre) and littermate controls (NAAVgfp or WTAAVcre) were subjected to 2/3 partial hepatectomy and analyzed 36 hours later.

(A) Immunofluorescence showing over-expression of Nampt in peri-portal and peri-central venous areas of NAAVcre mice. Inset shows an enlarged view of NAAVcre.

(B) Fasting blood glucose.

(C) Left: Photographs of regenerating livers. Right: Liver to body weight ratios.

(D) Left: Proliferating hepatocytes identified by EdU detected by immunofluorescence (green) and counterstained with DAPI (blue). Inset shows an enlarged view of NAAVcre. Right: Quantification of EdU-positive hepatocytes (n=5/group).

(E) Hepatic triglyceride content.

(F) Left panels: representative liver sections stained with H&E. Right panels: lipid accumulation by Oil Red O.

Error bars represent S.E.M. *, p < 0.05; **, p < 0.01; ***, p < 0.001.