Figure 7.

Complementary assay of Arabidopsis hsfa2 mutant with transformed FaHsfA2c. (a) Selection of heat‐tolerant transgenic lines. The experiments were performed as described in Figure 3a, except that 16 seeds for each line were grown on one petri dish and four petri dishes as replicates were treated at 45 °C for 3 h. Values indicate the total survival rate of plants in four petri dishes for each line. (b) Heat tolerance of wild‐type Arabidopsis, hsfa2 mutant and transgenic lines (35S:FaHsfA2c/hsfa2‐a and ‐b). The 30‐day‐old plants were treated at 45/40 °C (day/night) for 60 h and then recovered at 25 °C for 10 day. (c) FaHsfA2c expression levels in wild‐type Arabidopsis, hsfa2 mutant and transgenic lines. N.D., not detected. (d) Expression of FaHsfA2c‐GFP fusion protein in 35S:FaHsfA2c/hsfa2 transgenic lines. Five‐day‐old seedlings on agar plate were observed under stereo fluorescence microscope.