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. 2016 Nov 11;15(2):183–196. doi: 10.1111/pbi.12601

Figure 9.

Figure 9

Interaction proteins and molecular chaperone activity of OsASR5. (a) Y2H assay of OsASR5 interacting proteins. BD‐OsASR5 co‐transformed with AD empty vector is used as negative control. Three different concentrations of yeast cells were grown on control plate (‐Trp/‐Leu) and selective plate (‐Trp/‐Leu/‐Ade/‐His/X‐α‐gal). (b) BiFC assay for the in vivo interaction of OsASR5 with OsHSP40 and with OsFe(II) Oxy in tobacco epidermal cells (upper) and rice protoplast (lower). Bar, 10 μm. (c) OsASR5 and BSA (control) were not (−) or were (+) boiled at 100 °C for 30 min (n = 3). (d) LDH activity in the presence or absence of OsASR5 during cycles of freeze–thaw treatments (n = 3). Data are mean ± SE. ** indicates significant difference at P < 0.01 probability.