Abstract
The genome of infectious bursal disease virus consists of two segments of double-stranded RNA of 2.5 X 10(6) and 2.2 X 10(6) molecular weight. Polyacrylamide gel electrophoresis of purified virus resolved four structural polypeptides: VP-1 (90,000), VP-2 (41,000), VP-3 (35,000), and VP-4 (28,000). Peptide map comparisons of radioiodinated virion proteins indicated no precursor-product relationship between them. The possible relationship between the size of the virus genome and the number and sizes of different viral proteins is discussed.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dobos P., Hallett R., Kells D. T., Sorensen O., Rowe D. Biophysical studies of infectious pancreatic necrosis virus. J Virol. 1977 Apr;22(1):150–159. doi: 10.1128/jvi.22.1.150-159.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dobos P., Rowe D. Peptide map comparison of infectious pancreatic necrosis virus-specific polypeptides. J Virol. 1977 Dec;24(3):805–820. doi: 10.1128/jvi.24.3.805-820.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dobos P. Size and structure of the genome of infectious pancreatic necrosis virus. Nucleic Acids Res. 1976 Aug;3(8):1903–1924. doi: 10.1093/nar/3.8.1903. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dobos P. Virus-specific protein synthesis in cells infected by infectious pancreatic necrosis virus. J Virol. 1977 Jan;21(1):242–258. doi: 10.1128/jvi.21.1.242-258.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elder J. H., Pickett R. A., 2nd, Hampton J., Lerner R. A. Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein. J Biol Chem. 1977 Sep 25;252(18):6510–6515. [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Müller H., Scholtissek C., Becht H. The genome of infectious bursal disease virus consists of two segments of double-stranded RNA. J Virol. 1979 Sep;31(3):584–589. doi: 10.1128/jvi.31.3.584-589.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nick H., Cursiefen D., Becht H. Structural and growth characteristics of infectious bursal disease virus. J Virol. 1976 Apr;18(1):227–234. doi: 10.1128/jvi.18.1.227-234.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith R. E., Zweerink H. J., Joklik W. K. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. doi: 10.1016/0042-6822(69)90017-8. [DOI] [PubMed] [Google Scholar]
- Underwood B. O., Smale C. J., Brown F., Hill B. J. Relationship of a virus from Tellina tenuis to infectious pancreatic necrosis virus. J Gen Virol. 1977 Jul;36(1):93–109. doi: 10.1099/0022-1317-36-1-93. [DOI] [PubMed] [Google Scholar]