Figure 2.

a-g. Longitudinal representative histological sections of the distal metaphyseal region of femora (a-d). Histological assessment shows FPI + VEH rats (a) had a lower fraction of trabecular bone compared to SHAM + VEH rats (b). Whereas, there was a larger proportion of trabecular bone in FPI + SS rats (d) when compared to FPI + VEH rats (c; Goldner’s trichrome stain; original magnification 25 x). Histomorphometric analysis (e) shows a decreased trabecular bone volume fraction in FPI + VEH rats compared to SHAM + VEH rats however trabecular bone volume fraction was greater in FPI + SS rats compared to FPI + VEH rats. SS-treated rats had shorter femora (f) and wider growth plates (g) compared to VEH-treated rats. Treatment** indicates a significant main treatment effect. *p<0.05, **p<0.01, ***p<0.001.