Figure 3.
Correlation of 64Cu uptake on PET/CT with histological analysis of postmortem mouse muscular tissues. A. Increased 64Cu uptake by injured muscular tissues in mice with muscular injury caused by electroporation quantified by PET/CT after intravenous injection of 64CuCl2, compared with low 64Cu uptake by muscular inflammation induced by LPS and background 64Cu uptake by non-injured muscular tissues. B. Microscopic imaging of H&E stained postmortem mouse muscular tissue. Photo on left: non-injured mouse muscle showing normal morphology of myofibers (N denotes normal). Photo at middle: Muscular tissue injured by electroporation, showing degeneration and necrosis of myofibers and macrophagocytosis (highlighted by black arrows) with regenerating cells indicated by centrally located nuclei (highlighted by white arrows). Photo on right: Muscular tissue with inflammatory cell infiltration induced by LPS. h, hour; LPS, lipopolysaccharides; % ID/g, percentage of injected dose per gram.