Figure 4.

Urinary excretion of NKCC2 in sham and BUO rats. Urine samples were separated by SDS-PAGE (8.5%) and blotted onto nitrocellulose membranes. NKCC2 was identified using commercial polyclonal antibody as described in Section 2. The densitometric quantification of urine NKCC2 immunoblotting is expressed as the percentage of a ratio of arbitrary units relative to urinary creatinine concentration for each sample. Sham levels were set at 100%. Results are expressed as mean values ± SE. ^AP < 0.05 versus sham, ^BP < 0.05 versus BUO-1, ^CP < 0.05 versus BUO-2, and ^DP < 0.05 versus BUO-7.