. 2016 Dec 29;31(1):36–42. doi: 10.1111/jvim.14612

Table 1.

Specific primer sequences for real‐time polymerase chain reaction analysis with product sizes and optimal annealing temperatures

Target Gene	Accession Number	Direction	Sequence (5′ to 3′)	Product Size (Base Pair)	Annealing Temp. (°C)
Ob	NM_001003070.1	Forward	ACCGTATGGGTGTCCTTTATCCT	63	58
Ob	NM_001003070.1	Reverse	AGAGTGGCTCTGTGGTGTGAGA	63	58
Ob‐R	NM_001024634	Forward	CTTTTGCCTGCTGGAATCTC	143	58
Ob‐R	NM_001024634	Reverse	TTGCTCCAAAAGCAACAGTG	143	58
GAPDH	NM_001003142.2	Forward	CATTGCCCTCAATGACCACT	105	58
GAPDH	NM_001003142.2	Reverse	TCCTTGGAGGCCATGTGGAC	105	58

Ob, leptin; Ob‐R, leptin receptor; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase.