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. 2017 Jan 24;8:52. doi: 10.3389/fpls.2017.00052

FIGURE 2.

FIGURE 2

Identification of transgenic AtDREB1C Salvia miltiorrhiza plants via PCR. (A) PCR analysis of primary transformants using specific primers for 35S::AtDREB1C. Lanes 1 and 16, size markers; lanes 2–13, DNA from putative transformants; lane 14, untransformed control; lane 15, p35S::AtDREB1C. The 18S rRNA gene was used as a control. (B) PCR analysis of primary transformants using specific primers for RD29A::AtDREB1C. Lanes 1 and 16, size marker; lanes 2–13, DNA from putative transformants; lane 14, untransformed control; lane 15, pRD29A::AtDREB1C. The 18S rRNA gene was used as a control. (C) RT-PCR analysis of AtDREB1C expression in transgenic plants using under drought conditions. Lanes 1 and 16, size markers; lanes 2–7, DNA from PCR-positive p35S::AtDREB1C transformants; lanes 8–13, DNA from PCR-positive pRD29A::AtDREB1C transformants; lane 14, untransformed control; lane 15, p35S::AtDREB1C and pRD29A::AtDREB1C. The actin gene served as the internal control.