Table 1.

Primer Sequences used in triple gene plasmid construction.

Primer name	Primer sequences	Product
35SF	5′ CGCATTTAAATCTTAATTAATCCCCAGATTAGCCTTTTCAATTTCAG 3′	2X 35S promoter
35SR	5′ ATAGGATCCAGCTTGTCAGCGTGTCTCTCCAAATGAAATGAAC 3′
Cr1AcF	5′ CTAGGATCCAGCAGATCGAAATGGACAATAATCCGAATA 3′	Cry1Ac gene
Cr1AcR	5′ GCTAAGCTTCTAGATCATTCTAAAGTTGCAGTAACTG 3′
35STF	5′ GTTAAGCTTACCGTCACCGGTGTGAGGGAACTAG 3′	35S terminator
35STR	5′ TATGTCGACACCTAAGGTTCGGACGGTACGCTGAA 3′
2AbF	5′ TCTGGATCCACAAATCTCATGCAAGATATCC 3′	Cry2Ab gene and E9 terminator
G7R	5′ CAGAAGCTTCGCGTCGACGATCTTGAAAGAAATATAG 3′
CVMF	5′ CTCATTTAAATGAAGGTAATTATCCAAGATGTAGCATCAAG 3′	CVM promoter
CVMR	5′ TTCGGATCCTTCACCACAAACTTACAAATTTCTC 3′
EPSPSF	5′ GAAGGATCCGAAATGGCTCAAATAAACAACATGGCT 3′	EPSPS gene and E9 terminator
E9R	5′ ATTAAGCTTGGCGCGCCGATGTTTTACTCCTCATAT 3′