Figure 3.
Effect of miR508-3p on messenger RNA (mRNA) and protein abundance of microphthalmia transcription factor (MITF). (a) miR508-3p expression in melanocytes transfected with the miR508-3p expression plasmid. (b) MITF mRNA expression in melanocytes transfected with the miR508-3p expression plasmid. (c–e) MITF protein expression in melanocytes transfected with the miR508-3p expression plasmid analyzed using Western blot and immunocytochemical detection, respectively. Data are expressed as mean±SE from three replicates. ***P<0.001. NC=negative control.
