Figure 7.

Samd4 (sterile alpha motif domain containing protein 4) influenced chondrogenesis through mitogen-inducible gene 6 (Mig6). (a) Safranin O staining of tibia from 3-week-old wild-type (WT) and Samd4^PB/PBmale mice, showing the proliferation zone (white box) and the hypertrophic zone (black box). (b) Immunohistochemical staining of the indicated genes on the sections of tibia from 3-week-old Samd4^PB/PB mice and control WT mice. (c) Representative photographs of alcian blue staining of chondrocytes separated from WT and Samd4^PB/PB articular cartilage. (d, e) Analysis of the transcript levels of genes by quantitative PCR using RNA isolated from chondrocytes of WT and Samd4^PB/PB. (f and i) Representative photographs of alcian blue staining of chondrocytes differentiated from WT and Samd4^PB/PB articular cartilage and infected with lentivrus indicated, respectively. (g and h) Analysis of the transcript levels of genes by quantitative PCR using RNA isolated from chondrocytes of WT and Samd4^PB/PB that had been infected with the lentivirus-expressing enhanced GFP or Samd4. β-Actin, beta-actin. Values represent mean±s.d. (n=3 in panels (d, e, g and h) for each genotype). P-values were obtained from t-tests with paired or unpaired samples, *P<0.05. Bars=100 μm in panels (a and b) and 1 mm in panels (c, f and i).