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. 2017 Jan 24;7:41125. doi: 10.1038/srep41125

Figure 3. Anti-HLA antibody-induced ADCC is binding strength-dependent.

Figure 3

IL-15-activated NK cells were cultured alone (medium) or with hCPC or IFNγ-hCPC (n = 6) in the presence of declining concentrations of anti-HLA-I W6/32 or 10 μg/ml of W6/32-F(ab’)2. (a) % CD137-positive NK cells determined by flow cytometry (upper panel). % CD137-positive NK cells observed for each antibody concentration in hCPC or IFNγ-hCPC plotted as function of respective MFIs (lower panel). (b) % NK cell-mediated lysis evaluated as percentage of 7AAD-positive hCPC or IFNγ-hCPC (upper panel). % CD107a-positive NK cells plotted as function of % NK-mediated lysis (lower panel). Results are presentenced as mean values ± SD from three different experiments done with each hCPC. Statistical analyses were performed using One-Way Analysis of Variance (ANOVA)-Kruskal–Wallis test-dunn’s multiple comparison (GraphPadPrism Software). P < 0.01 and P < 0.001 compared to NK + hCPC alone.