Table 3. Distribution and diversity of the gene clusters involved in the biosynthesis of natural products of the studied Planktothrix.
| RiPPs |
NRPS |
NRPS/PKS hybrid |
PKS |
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| mdn | pat-like | osc | pag | RiPP1 | aer | apt | oci | PNL2 | PNL3 | mcy | mic | has-like | PNL1 | PHL1 | lum | tto | PPL1 | PPL2 | PPL3 | |
| Size (kb) | 7,1–7,3 | 11,1 | 8,2–11,8 | 9,3–11,4 | 3,4 | 22,8–25,7 | 23,7 | 27,5–32,3 | 11 | 11,3 | 49,3–51,1 | 34,5 | 43,2 | 25,1 | 28 | 89,5 | 92,8 | 11,7 | 8 | 6,2 |
| PCC 7821 | Ref. | — | Ref. | Ref. | — | Ref. | Ref. | Ref. | 100£ | — | Ref. | 100£ | — | — | — | — | — | — | — | — |
| PCC 7805 | 93 | — | 97 | 96 | — | 82 | partE | 91 | — | — | — | — | — | 96£ | — | — | — | — | — | — |
| PCC 11201 | 92 | — | 83 | 92 + partABC | — | 54 | — | 55 | — | — | 73 | — | — | — | — | — | 76§ | — | — | — |
| PCC 9214 | — | — | — | — | — | — | partBCD | — | — | Ref. | — | — | — | — | Ref. | — | — | — | Ref. | Ref. |
| PCC 8927 | — | — | — | — | Ref. | — | — | — | — | — | — | — | 66$ | — | — | — | — | Ref. | — | — |
| PCC 9631 | — | 91* | — | partGEF | — | — | — | — | — | — | — | — | — | — | — | Ref. | — | — | — | — |
Ribosomally synthesized and post-translationally modified peptides (RiPPs), non-ribosomal peptide synthetase (NRPS), polyketide synthase (PKS) and NRPS/PKS hybrids were compared by amino acid identity (% AAI) of NRPS/PKS/cyanobactin protease to a reference Planktothrix strain (Ref.), Microcystis sp. PCC 9432 (*), Anabaena sp. SYKE748A ($), Planktothrix prolifica NIVA-CYA 98 (£) and Scytonema sp. PCC 10023 (§). Incomplete gene clusters are indicated with the conserved genes (part). The biosynthetic gene clusters of aeruginosin (aer), anabaenopeptin (apt), cyanopeptolin (oci), luminaolide (lum), microcyclamide (mca), microcystin (mcy), microginin (mic), microviridin (mdn), oscillatorin (osc), prenylagaramide (pag), tolytoxin (tto) were detected as well as one like hassallidin (has-like), and another one like cyanobactin (pat-like), and other Planktothrix gene clusters not corresponding to know molecules named Planktothrix NRPS-like (PNL), Planktothrix PKS-like (PPL) and Planktothrix hybrid-like (PHL).